A new assay for indirectly detecting nitric oxide in serum was established. Nitrate was reduced to nitrite by activated spongy cadmium with copper ion on a microscale chromatographic column, then the nitrite was measured by the Griess reaction. 采用铜离子活化海绵镉微型柱层析法将血清中的硝酸盐(NO-3)还原为亚硝酸盐(NO-2),再通过Griess反应测定NO-3/NO-2总量以反应体内一氧化氮(NO)水平,从而建立血清NO间接测定新方法。
Application of the Griess reaction in the study of internal indicator for diazotization determination 利用GRIESS反应做重氮化法含量测定内指示剂的研究
Objective: To observe the effect of mongolian medicine Naru-3 on the content of Nitric Oxide ( NO) in the heart, liver and kidney of rat. Methods: Griess reaction is used to determine the content of NO in the homogenate. 目的:探讨蒙药那如-3对大鼠心脏、肝脏和肾脏中一氧化氮(NO)含量的影响。方法:采用改良的Griess法测定心、肝和肾匀浆中NO含量。
The levels of IFN-γ and IL-12 in sera were measured by ELISA kits, and level of NO in spleen cell suspensions was assayed by Griess reaction. 应用ELISA试剂盒检测小鼠血清中IFNγ和IL12水平,并通过Griess方法检测脾细胞培养上清中NO含量。
Using an in situ collagenase-pronase E perfusion technique, Kupffer's cells were isolated from hepatocytes in rat immunological hepatic injury, and nitric oxide ( NO) production in culture supernatant was measured by the method of the Griess's reaction. 采用胶原酶-链霉蛋白酶灌流法对免疫性肝损伤大鼠肝实质细胞与枯否细胞进行分离与原代培养,Griess反应法检测细胞培养上清中一氧化氮(NO)生成量的变化。
Methods: NO accumulation in the cultured supernatant was measured with the Griess's reaction, and intracellular cGMP level with radioimmunoassay. 方法:用Griess反应法检测细胞培养上清NO含量,放射免疫分析法检测细胞内环磷酸鸟苷(cGMP)水平。
The levels of parasitemia were observed during infection by Giemsa stain of thin blood smear, and the levels of IFN-γ and NO in culture supernatants of splenocytes from DBA/ 2 mice were also measured by ELISA kits and Griess reaction, respectively. 薄血膜染色,计数红细胞感染率;用ELISA试剂盒和Griess实验分别检测DBA/2小鼠脾细胞培养上清中IFN-γ和NO含量。
To investigate the inducible effects of IFN γ on NO synthesis of macrophages during the infection of Plasmodium yoelii, we observed the NO levels in supernatants from splenocytes and macrophages of infected mice with Griess reaction. 为探讨疟原虫感染过程中IFNγ对巨噬细胞合成NO的诱导作用,我们应用Griess反应检测了约氏疟原虫感染小鼠脾细胞和MΦ培养上清中NO的分泌情况。
Methods: By using ELISA and Griess reaction, we investigated the effects of cytokines and NO on parasitemia during the early phase of infection. 方法:通过ELISA和Griess反应,观察疟原虫感染早期宿主体内细胞因子和NO对红内期虫体血症的影响及其效应。
Serum nitrate ( NO 3-), the end products of NO and plasma ET were measured by activated cadmium reducer combined with Griess reaction and radioimmunoassay respectively before and after infusion of hANP or placebo. Blood pressure changes were observed. 应用活化镉还原剂与Griess反应结合法及放射免疫分析法分别测定用药前后血清NO代谢终产物硝酸基(NO3-)及血浆ET水平,并观察血压变化。
Concentrations of NO in cell supernatants were measured by the Griess reaction. Griess反应检测脾细胞培养上清中一氧化氮(NO)含量。
The level of serum NO was measured by the Griess reaction after reduction with nitrate reductase. Results Compared with the control, maternal as well as umbilical serum LPO and NO in FGR group were significantly higher ( P < 0.01 and P < 0.001 respectively). 结果FGR组患者血LPO和NO水平与对照组相比均显著升高(P<0.01),其新生儿脐血LPO和NO水平也显著升高(P<0.001)。
Methods: NO output, iNOS ( inducible nitric oxide synthase) activity and reduced glutathione ( GSH) level were determined by Griess reaction, fluorimetry and DTNB colorimetry respectively. 方法:采用Griess反应、荧光法和DTNB比色法分别测定不同剂量的PPS作用小鼠腹腔巨噬细胞的NO生成量、诱导型一氧化氮合酶(iNOS)活性及细胞内还原性谷胱甘肽(GSH)含量的变化。